There are several manual methods to separate peripheral blood mononuclear cells (PBMCs) from leukapheresis products, but most of them are highly user dependent and laborious. The Gibco™ CTS™ Rotea™ Counterflow Centrifugation System can be used to automate a variety of methods to separate PBMCs in a closed environment.
In this application note, you’ll see effective and consistent PBMC separation from the CTS Rotea system across all tested methods compared to a manual density gradient centrifugation method.
Test methods include:
- Ficoll™ solution
- With ACK (ammonium-chloride-potassium) lysis buffer
- Without lysis buffer